Analysts interested in organic molecules like dissolved organic matter or pharmaceuticals should consider quantifying organic molecules with the High Performance Liquid Chromatography (HPLC) Instrument. There are three different HPLC systems in the engineering laboratories. All HPLC’s measure low level organic compounds in surface water, groundwater, and drinking water. The HPLC With Fluorescence Detector (FLD), Diode Array Detector (DAD), and Total Organic Carbon (TOC) Analyzer instrument can separate molecules from each other based on physicochemical properties like molecular size, polarity, and electrical charge depending on the solvents used to carry the sample and the type of separator column. The current setup uses a size exclusion column column (SEC). After the molecules are separated, the HPLC system quantifies organic molecules with a diode array detector (DAD), fluorescence detector, and total organic carbon detector. Each detector uses software to create peaks for each molecule, which can be quantified by integrating and comparing peak areas of samples to peak areas of calibration standards.
Agilent HPLC 1260 Series with DAD, FLD, and TOC Analyzer
