Extended Depth of Field Microscopy

EDF Microscopy: Feather at 3x

Images of a tilted feather at three times magnification. These images were taken using a traditional imaging system. The above image is focused on the front of the feather. By moving your mouse over the above image, you can see an image which is focused on the back of the feather.

The above image is an intermediate raw image of the feather taken using the Cubic Phase Plate (CPP). By moving your mouse over the above image, you can see the filtered image, after digital post-processing.

EDF Microscopy: Tissue at 3x

Images of two stacked slides under three times magnification. These images were taken using a traditional imaging system. The above image is focused on the front slide, which contains muscle tissue. By moving your mouse over the above image, you can see an image which is focused on the back slide, which contains skin tissue.

The above image is an intermediate raw image of the two stacked slides taken using the Cubic Phase Plate (CPP). By moving your mouse over the above image, you can see the filtered image, after digital post-processing. 

EDF Microscopy: Salt Grains at 10x

Images of a salt crystals at ten times magnification. These images were taken using a traditional imaging system. The above image is focused on the top of the salt crystals. By moving your mouse over the above image, you can see an image which is focused on the bottom (respectively) of the salt crystals.

The above image is an intermediate raw image of the salt crystals taken using the Cubic Phase Plate (CPP) inserted into the aperature stop. By moving your mouse over the above image, you can see the filtered image, after digital post-processing.

EDF Microscopy: Metallurgical Microscope

Images of a materials testing object taken with a Bausch & Lomb MicroZoom metallurgical microscope. The 25X objective of the microscope was used for these images. The materials testing object is used to analyze stress/strain properties of the material. The first two images were taken using a standard metallurgical microscope. The second set of images were taken using the same microscope fitted with the extended depth of field (EDF) cubic phase mask. The last set of images are the EDF images from the second set after digital post-processing.

 

Above is an in focus image of the test object using a standard metallurgical microscope. Below is the same test object moved 60 microns below the focal plane.

The images below were taken with the same metallurgical microscope after modification by placing a cubic phase mask in the back aperture of the objective.

 

Above is an image of the test object taken using the cubic phase mask modified microscope, with the object in the origical focal plane. Below is the same test object, again, moved 60 microns below the original focal plane but taken with the cubic phase mask modified microscope.

 

Above and below are the cubic phase mask modified microscope images of the test object shown in the previous section but after digital post-processing.