In this publication, several new genetically encoded sensors for Zinc (II) are presented based upon fluorescence resonance energy transfer (FRET). Sources and sinks for Zinc (II) are explored within the cell, including the cytosol, plasma membrane, and mitochondria. Localized probes reveal that mitochondria contain an elevated pool of Zinc (II) under resting conditions that can be released into the cytosol upon glutamate stimulation of hippocampal neurons. Results suggests that mitochondria serve as a source of and a sink for Zinc (II) signals under different cellular conditions.
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The Palmer Lab at University of Colorado Boulder