Lab News

Dawn Schaefer (Colorado School of Mines) and Emily Gibson report a simple fabrication technique to create all silicon/glass microfluidic devices using femtosecond laser ablation and anodic bonding. In a first application, they constructed a cell counting device based on small angle light scattering. The counter featured embedded optical fibers for...

Schuyler successfully defended his Ph.D. and has accepted a position as a post-doctoral associate with Dr. Jennifer Lippincott-Schwartz at the NIH in Maryland.

Featured in the news and views section of Nature Structural and Molecular Biology and published in Nature Methods, Schuyler uses a GFP complementation assay to tag effectors of the type-III secretion system in gram-negative bacteria, thereby allowing real-time localization and imaging of the effectors in the host cell throughout the...

The connection between presenillin-1, calcium regulation, and Alzheimer’s disease may provide the key to understanding the long-observed, but poorly understood, link between Alzheimer’s disease and Calcium (II) dysregulation. In this study, Janet examined seven familial Alzheimer’s causing mutations in presenillin-1 to define how they influence ER calcium levels and dynamics...

Schuyler performed an in situ selection of a randomized amino acid linker library (10.2 million peptides) flanking three tandem optimized TC repeats. Read the article here .

In this publication, several new genetically encoded sensors for Zinc (II) are presented based upon fluorescence resonance energy transfer (FRET). Sources and sinks for Zinc (II) are explored within the cell, including the cytosol, plasma membrane, and mitochondria. Localized probes reveal that mitochondria contain an elevated pool of Zinc (II)...

Presenting at Janelia Farm's 'Novel Approaches to Bioimaging II' Conference, Kevin was asked to give a short talk regarding his recent work involving microfluidic based optimization of red-fluorescent proteins.