MACRO, Stereo, Brightfield (RGB color, epifluorescence)
FRANK - Zeiss Discovery.V12 fluorescent stereo (Porter B059)
Light Sources:
- X - Cite 120
- Fostec Fiber Optic halogen illumination
Standard fluroescent filter configurations:
- GFP, AzamiGreen, Emerald, etc.
- mRFP, mCherry, TdTomato, etc.
• 1X Zeiss NA0.XX objective
• 8X - 100X via motorized optical zoom
• selectable display to indicate field size diameter, resolution
Nikon Digital Sight CCD camera (DS-Qi; 6.45 um x 6.45 um pixel)
- Fluorescent imaging of GFP and RFP and their related probes over a magnification range.
- Halogen illumination via Fostec Fiber Optic.
- NIS Elements BR 5.20.02 for fluorescence imaging (monochrome)
PEPPER - Nikon TiU widefield with RGB color brightfield (Porter B047A)
Light Sources:
- X - Cite 120
- Halogen illumination for brightfield and DIC
Standard filter configurations:
Semrock BrightLine quad Sedat
- DAPI, Hoechst, etc.
- Alexa488, FITC, GFP, etc.
- TRITC, Alexa555, 568, 594, mRFP, mCherry, TdTomato, etc.
- Alexa647, Cy5, etc.
Standard objectives:
- 2x 0.10NA Plan Apo WD 8.5 (mm)
- 4x 0.13NA Plan Fluor WD 17.2 (mm)
- 10x 0.45NA Plan Apo DIC N1 WD 4 (mm)
- 20x 0.50NA Plan Fluor WD 2.1 (mm)
- 40x 0.60NA Plan Fluor WD 1.8 (mm)
- 100x 1.30NA Plan Fluor Ph3 OIL WD 0.20 (mm)
Optional objectives:
- 60x 1.40NA Plan Apo VC λ OIL WD 0.13 (mm)
- More (please inquire)
- CoolSNAP HQ2 monochrome camera for fluorescence
- Nikon DS-Fi3 24 bit high definition color camera for color brightfield (e.g. eosin, hematoxylin, crystal violet. etc.). This camera uses a CMOS chip with 5.9 megapixels.
ASI MS-2000 XYZ motorized stage. Sample holders for standard slide, 35 and 60 mm dishes, and multiwell plates. DIC possible at 10x, and 60x. Brightfield color RGB imaging. Multi-point imaging. Montaging with stitching. Live cell imaging with TOKAI-HIT Cell Incubator; 1 X 35 (mm) dish or chamber slide (coverslip bottom). Rapid 4 color imaging via quad Sedat filter set (Semrock BrightLine).
MACRO - Olympus MVX10 MacroZoom fluorescent & color (Porter B059)
- Built in high-contrast white LED in base for transmission imaging
- CoolLED pE-300White: 400, 488, 561 nm LEDs
- ET-DAPI/FITC/TxRed triple Sedat filter set
Objectives (note, 2 position nose piece):
- Olympus 0.63X MVXPLAPO NA0.15 WD87mm (max field of view 55mm)
- Olympus 1X MVXPLAPO NA025 WD65mm (max field of view 34.9mm)
- Olympus 2X MVXPLAPO NA0.50 WD20mm (max field of view 17.6mm)
Zoom body:
- 0.63 - 6.3X
System magnifier:
- 2X
Effective magnification (to eyes; 10X less to camera when using 1X coupler and no system magnifier):
- Total system, 4 - 250X
- 0.63X objective, 4 - 80X
- 1X objective, 6.3 - 125X
- 2X objective 12.5 - 250X
Color RGB camera:
- Olympus DP23, 1x1.8 inch 6.4MP (3088 x 2076 pixels) color CMOS, 2.4 micron x 2.4 micron pixels
Monochrome camera:
- Hamamatsu ORCA Spark 2.3MP 1`920 x 1200 pixels) CMOS, 5.86 x 5.86 micron pixels
System features:
- Sytem Macro and stereo viewing modes; macro maximizes brightness, stereo splits the light path to enhance depth of field
- Color camera is mounted with 0.63X adapter to maximize field size
- Monochrome camera is mounted at 1.0X distance, no glass to maximize brightness
- 2 position nose piece allows 2 objectives to be mounted simultaneouly
- Diaphragms to adjust brightness for both white light and fluorescece
- Triggered LED allow efficient blue, green, red imaging
- Customizable intensity for each LED to limit photobleaching
Capabilities:
- Base includes brightfield high-contrast, brightfield oblique, darkfield and blocking optics
- Time-lapse microscopy
- Multi-channel fluorescence
- Fiber optic white light (Fostec) for reflected light imaging
Olympus cellSens Standard version 4.2
