University Of Colorado Denver
iPS Cells
Isolation of Primary Fibroblast from Skin Biopsy or other sources
iPS Cell Generation with Sendai and Lenti Viruses
Immunocytochemistry Staining for Pluripotency Markers
QRT-PCR and RT-PCR for Pluripotency Markers
Embryoid Body Formation and Differentiation
Mycoplasma Testing of iPSC Line
Adapting iPSC to Feeder-Free Growth Conditions
Transgenic and Gene Targeting
Gene Targeting in ES Cells
Karyotyping
Removal of floxP or FRT-flanked DNA from the ES cells genome
Blastocyst Injection
Pronuclear Injection
ZFN, TALEN and CRISPR-mediated gene editing in mouse embryos
Cryopreservation of embryos and sperm
In vitro Fertilization
Embryo Rederivation