Imaging Systems

Typhoon 5

Please Typhoon 5 imager and monitor acknowledge SIP (RRID: SCR_018986) and the NIH funding (1S10OD034218-01) in publications and presentations ( posters, talks) if you use the Typhoon 5.

The Typhoon 5 is a multi-mode, high-resolution, high-sensitivity imaging platform that produces digital images of radioactive, ﬂuorescent, or chemiluminescent samples. It has blue (488 nm), green (532 nm), red (635 nm), near IR (685nm) and far IR (785nm) excitation wavelengths and a wide choice of emission filters (IP, P 390BP, Cy2 525BP20, Cy3 570BP20, Cy5 670BP30, IRshort 720BP20, IRlong 825BP30, Cy2 LPB515, Cy3 LPG550, Cy5 LPR660) enable imaging of an extensive variety of fluorophores. Automated multi-colour fluorescence scanning allows multiplexing of multiple targets in the same sample. It can be used to scan phospho storage screens, gel sandwiches, agarose and polyacrylamide gels, membranes, microplates and even microarrays.

IQ 800

Please acknowledge SIP (RRID: SCR_018986) and the NIH funding (R35GM136392-04S1) in publications and presentations (posters, talks) if you use the IQ 800 imager.

Th ImageQuant™ 800 imager from Cytiva/Fujifilm features a sensitive 8.3-megapixel high-resolution Peltier-cooled CCD detector with an F 0.74 lens, providing high-resolution imaging of very close bands. The IQ 800 is versatile, supporting chemiluminescence, fluorescence, and colourimetric imaging for various samples, including gels, membrane blots, multiwell plates, and petri dishes. Its improved optics, combined with the new SNOW™ (signal-to-noise optimization watch) detection mode, enhance both sensitivity and image quality.

Light sources are Epi-blue: 460 nm, Epi-green: 535 nm, Epi-red: 635 nm, Epi-IR short: 660 nm, Epi-IR long: 775 nm, Epi-UV: 365 nm, Epi-white: 470 to 656 nm and Trans-white. Filters available are Cy2: 525BP20, Cy3 (UV): 605BP40, Cy5: 705BP40, IR short: 715BP30, IR long: 836BP46.

All three imaging systems are easy and intuitive to use. It is ok for new users to be trained by someone in their lab, who uses them routinely. Users can also contact Dr. Erbse for help and training on the imaging systems.

Users can sign up for instrument time on EMS. Users must be connected via VPN, if not on campus. After connecting, users can log in with their Identikey username and password. Detailed step-by-step instructions for the sign-up process can be found here. If you need help, please reach out to the SIP director Dr. Erbse. In addition, users must sign the Excel log sheets on the desktops of the PCs to document their instrument usage.

The imagers are located in C381

Regular user groups are expected to opt into the pool and pay an monthly share that goes towards the general up keep of the Shared Instruments Pool. For detailed information please contact Dr. Annette Erbse.
Users are expected to provide all consumables specific to their experiment.
Groups must pay for any parts or repairs due to damage by neglect or carelessness (If you break it - you bought it!).
Cost for repairs/parts/services resulting from normal wear will be split between all user groups.
Please Note - Since repairs/service from normal wear might not be necessary every year, depending on use, these costs might come quite some time after use.
For more infromation see Usage Policies.

Users must clean the trays and the white insert after use.
Bring water, Ethanol and Kim wipes for cleaning.
Exposures longer than 30 min should not be done during normal working hours. Please time your experiments so that you can do them early or late in the day.
Do not cut on the glass tray.
Do not scratch the black tray with metal tweezers. Use plastic tweezers.

Users must clean the stages after use, especially the glass fluorescence stage.
Users must bring water, Ethanol and Kim Wipes for cleaning (only absolute 100% alcohol should be used on the glass stage). Lower-quality EtOH can leave fluorescent contaminations.
The large, mounted phospho storage screens can only be used with the Dige stage, please ask for help.
Glass plates should never be placed directly on the glass stage. Use the stage with the adjustable frame to hold your glass plates.
Do not cut on the glass stage.

The Shared Instruments Pool depends on acknowledgements to thrive. For more information on how to acknowledge the Shared Instruments Pool and/or its personnel, click here or go to the acknowledgement tab.

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Imaging Systems

Typhoon 5

IQ 800