Instrument/ServiceType:

Spinning Disk Confocal Microscope with FRAP and photo-activation

Make/ Model:

Nikon Inverted Spinning Disk Confocal Microscope

Instrument Description:

The inverted Nikon spinning disk confocal microscope is equipped with four laser lines (405, 488, 561, and 647 nm), 4x-40x air objective lenses, and 60x NA1.40 and 100x NA1.45 oil objective lenses. Dual EM-CCD cameras for widefield (Hamamatsu ImageEM) and confocal imaging (Andor 897) are used.  A separate high-intensity 405 nm laser can be used for photobleaching, photoconversion, and photoactivation imaging.  A Tokai-Hit incubation chamber with single 35mm dish or a multi-well chamber slide can be used on this microscope for live imaging. This microscope can be used for a wide range of applications and is particularly well suited to fast image acquisition of dim samples, live imaging, and large area scanning. 

Instrument Website URL:

https://www.colorado.edu/lmcf/microscopes/confocals

Primary Contact:

James Orth

Email id: james.orth@colorado.edu

Mobile Number: 303-492-5955 /303-492-5057

Instrument Location:

Porter B047A