Instrument/Service Type:
Multi-photon microscopy, IR
Make/Model:
Olympus FVMPE-RS twin laser
Instrument Description:
A dedicated Olympus multi-photon microscope. Two separate, tunable, pulsed IR lasers, a Spectra Physics Insight X3 (680-1300 nm) and Spectra Physics MaiTai eHP DeepSee (690-1040 nm). Four PMT detectors. Galvanometer and resonance scanning. Multi-photon objectives: 5x NA 0.10 air WD 20 mm, 10x NA 0.60 water immersion WD 3.3 mm, 25x TruResolution water immersion NA 1.05 WD 2.0 mm, and 60x water immersion NA 1.10 WD 1.5 mm. A 20x air, NA 0.60 WD 8.2 mm is available for GRIN lens work. Olympus Fluoview acquisition software, motorized Prior Z-Deck stage allowing for multipoint, tiling, Z-series time-lapse imaging, etc. Other features include auto-alignment, beam-shaping for improved deep focus, and BrightZ power-compensation. A sample warming pad (Stoelting) and isoflurane anesthesia is available for imaging small animals. Ideal for simultaneous multi-color, deep, in situ imaging of excised or live samples, including tissues and whole animals.
Keywords for Instrument:
Multi-photon, in situ, intravital, IR, deep tissue, GRIN lens
Instrument Website URL:
https://www.colorado.edu/lmcf/multi-photon
Primary Contact:
James Orth
Email: james.orth@colorado.edu
Phone: 303-492-5955 or 303-492-5057
Instrument Home Department/Institute:
MCDB
Instrument Home Facility:
Light Microscopy Core Facility
Instrument Location:
Porter B051
Available to university users?
Yes
Available to external, academic and national lab users?
Yes